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Undergraduate Biology Biology Learning Labs SUNY at Stony Brook Stony Brook, NY 11794-5110 631-632-8530 Fax 631-632-1347
| BIO 365 - Biochemistry Lab Fall 2000
Course Description
The Biochemistry Laboratory is a series of laboratory experiments to complement the lecture series Biology 361-362. Topics include introduction of techniques for isolation of cellular organelles, genetic fingerprinting, enzyme kinetics, and recombinant DNA. The course consists of fours hours of laboratory/discussions per week.
Pre- or co-requisites: Biology 310 or 361
Course Syllabus
| Date | Exp. # | Description | Students per Exp. | Quiz/Lab Reports |
| Sept 5-7 | Introduction | Orientation |  |  |
| Sept 12-14 | I, week 1 | Cellular Constituents: cell number, extration, TLC chromatography | 2 |  |
| Sept 19-21 | I, week 2 | Cellular Constituents: DNA, RNA, Protein | 2 | Quiz 1 |
| Sept 26-28 | II, week 1 | Genetic Fingerprinting | 1 | Report 1 |
| Oct 3-5 | II, week 2 | Genetic Fingerprinting | 1 | Quiz 2 |
| Oct 10-12 |  | NO CLASS |  |  |
| Oct 17-19 | III, week 1 | Catalase | 2 | Report 2 |
| Oct 24-26 | III, week 2 | Catalase | 2 | Quiz 3 |
| Oct 31-Nov 2 | IV, week 1 | Recombinant DNA: Restriction digest, Gel electrophoresis, Ligation | 4 | Report 3 |
| Nov 7-9 | IV, week 2 | Recombinant DNA: Transformation of E.coli | 4 |  |
| Nov 14-16 | IV, week 3 | Recombinant DNA: Plasmid mini preperation, Restriction digest, Electrophoresis | 4 |  |
| Nov 21-23 |  | NO CLASS Thanksgiving |  |  |
| Nov 28-30 |  | Hand in last lab report, take last quiz |  | Report 4 |
| Dec 5-7 |  | Lab reports returned, check out of lab |  |  |
Grading
Laboratory reports should be handed to the instructor for each section one week after completion of the laboratory. Ten (10) points will be deducted for each day a lab report is late. Grades will be curved in each section to ensure consistency between sections. The course will be graded as follows:
| Laboratory Reports | 175 points (I-IV) | = 700 points |
| Laboratory Performance | 100 points | = 100 points |
| Quizzes | 50 points for 1-4 | = 200 points |
| TOTAL |  | = 1000 points |
Teaching Assistants
| Teaching Assistant | Section | Lab day & time | Room | Office Hour | Email |
| Anagha Matapurkar | 1 | Tuesday
8:20 - 12:20 | 371 | Tuesday
14:00 - 15:00 | amatapur@ic.sunysb.edu |
| Asra Malizkay | 2 | Tuesday
8:20 - 12:20 | 371 | Tuesday
10:00 - 11:00 | amalizka@ic.sunysb.edu |
| Marian Nyako | 3 | Tuesday
17:00 - 21:00 | 311 | Monday
16:30 - 17:30 | mnyako@ic.sunysb.edu |
| Vedell Louis-Jeune | 4 | Tuesday
17:00 - 21:00 | 311 | Monday
16:00 - 17:00 | vedell@yahoo.com |
| Lisa Martin | 5 | Wednesday
13:00 0 17:00 | 311 | Monday
13:00 - 14:00 | liamarti@ic.sunysb.edu |
| Tuzer Kalkan | 6 | Wednesday
13:00 0 17:00 | 311 | Monday
13:00 - 14:00 | tuzerka@yahoo.com |
| Ling Liu | 7 | Thursday
8:20 - 12:20 | 371 | Thursday
14:30 - 15:30 | linliu@ic.sunysb.edu |
| Anna Zinovyeva | 8 | Thursday
8:20 - 12:20 | 348 | Monday
12:00 - 13:00 | zinovyeva@hotmail.com |
Scientific Links
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