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The Fetcho Lab
Neurobiology & Behavior
Life Science Bldg.
SUNY at Stony Brook
Stony Brook, NY 11794-5230
631-632-9047
FAX 631-632-6661
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Melissa Bishop/DoIT
Last Modified 11/18/99 04:19:00 PM EST
Red means "Go!"
The above animation shows three CiD interneurons which are active during an escape response. So that changes in fluorescent intensity can be more easily visualized, the cells are shown in pseudo color - with red indicating greater fluorescent intensity and blue indicating less. The cells start off green-to-yellow but turn red with the escape response (coinciding with the movement). They then slowly fade through orange towards yellow again.
Methods
The CiD cell population was labeled by pressure injecting a 50% solution of Calcium Green Dextran (CGD; Molecular Probes Inc. Eugene, OR) into dorsal spinal cord . The cells above were visualized using a Zeiss Axiovert 100TV fluorescence microscope with a 63X objective in conjunction with a Macintosh based imaging system.