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The Fetcho Lab
Neurobiology & Behavior
Life Science Bldg.
SUNY at Stony Brook
Stony Brook, NY 11794-5230
631-632-9047
FAX 631-632-6661

State University of New York at Stony Brook
Site Maintained by
Melissa Bishop/DoIT
Last Modified 11/18/99 04:16:05 PM EST

Calcium Imaging


Because larval zebrafish are transparent, labeled neurons can easily be viewed under fluorescence and confocal microscopes. Calcium Green Dextran (CGD) is a fluorescent, calcium sensitive dye which increases in fluorescence intensity with increases in cytosolic calcium concentration. Intracellular calcium concentration increases in electrically active neurons, so labeled neurons become brighter when they are active during a behavior. Using CGD, we can monitor the activities of labeled neurons in the intact fish during a behavior by literally watching which cells light up.

CiD Cell Activity During an Escape
(Circumferential Descending Interneurons)
Click to run animation
(417K)
(Click Image to run animation)